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Quantitative RT-PCR assay for high-throughput screening (HTS) of drugs against the growth of Cryptosporidium parvum in vitro

Our laboratory has previously developed a qRT-PCR assay to assess drug efficacy on the growth of Cryptosporidium parvum in vitro by detecting the levels of parasite 18S rRNA. This approach displayed up to four orders of magnitude of linear dynamic range and was much less labor-intensive than the tra...

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Bibliographic Details
Published in:Front Microbiol
Main Authors: Zhang, Haili, Zhu, Guan
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2015
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4585199/
https://ncbi.nlm.nih.gov/pubmed/26441920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2015.00991
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