Application of Quantitative Real-Time Reverse Transcription-PCR in Assessing Drug Efficacy against the Intracellular Pathogen Cryptosporidium parvum In Vitro

We report here on a quantitative real-time reverse transcription-PCR (qRT-PCR) assay for assessing drug efficacy against the intracellular pathogen Cryptosporidium parvum. The qRT-PCR assay detects 18S rRNA transcripts from both parasites, that is, the cycle threshold for 18S rRNA from parasites (C(...

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Detalhes bibliográficos
Main Authors: Cai, Xiaomin, Woods, Keith M., Upton, Steve J., Zhu, Guan
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Analytical Procedures
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1280145/
https://ncbi.nlm.nih.gov/pubmed/16251280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.49.11.4437-4442.2005
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