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Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells

Many key regulatory proteins in bacteria are present in too low numbers to be detected with conventional methods, which poses a particular challenge for single-cell analyses because such proteins can contribute greatly to phenotypic heterogeneity. Here we develop a microfluidics-based platform that...

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Bibliografski detalji
Izdano u:Nat Commun
Glavni autori: Okumus, Burak, Landgraf, Dirk, Lai, Ghee Chuan, Bakhsi, Somenath, Arias-Castro, Juan Carlos, Yildiz, Sadik, Huh, Dann, Fernandez-Lopez, Raul, Peterson, Celeste N., Toprak, Erdal, El Karoui, Meriem, Paulsson, Johan
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4873973/
https://ncbi.nlm.nih.gov/pubmed/27189321
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms11641
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