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Segregation of molecules at cell division reveals native protein localization
We introduce a non-intrusive method exploiting post-division single-cell variability to validate protein localization. The results show that Clp proteases, widely reported to form biologically relevant foci, are in fact uniformly distributed inside Escherichia coli cells, and that many commonly used...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3779060/ https://ncbi.nlm.nih.gov/pubmed/22484850 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1955 |
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