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Quantification of very low-abundant proteins in bacteria using the HaloTag and epi-fluorescence microscopy

Cell biology is increasingly dependent on quantitative methods resulting in the need for microscopic labelling technologies that are highly sensitive and specific. Whilst the use of fluorescent proteins has led to major advances, they also suffer from their relatively low brightness and photo-stabil...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Lepore, Alessia, Taylor, Hannah, Landgraf, Dirk, Okumus, Burak, Jaramillo-Riveri, Sebastián, McLaren, Lorna, Bakshi, Somenath, Paulsson, Johan, Karoui, M. El
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6536506/
https://ncbi.nlm.nih.gov/pubmed/31133640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-44278-0
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