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Quantification of very low-abundant proteins in bacteria using the HaloTag and epi-fluorescence microscopy

Cell biology is increasingly dependent on quantitative methods resulting in the need for microscopic labelling technologies that are highly sensitive and specific. Whilst the use of fluorescent proteins has led to major advances, they also suffer from their relatively low brightness and photo-stabil...

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Bibliografske podrobnosti
izdano v:Sci Rep
Main Authors: Lepore, Alessia, Taylor, Hannah, Landgraf, Dirk, Okumus, Burak, Jaramillo-Riveri, Sebastián, McLaren, Lorna, Bakshi, Somenath, Paulsson, Johan, Karoui, M. El
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2019
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6536506/
https://ncbi.nlm.nih.gov/pubmed/31133640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-44278-0
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