Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli

Robust gene circuit construction requires use of promoters exhibiting low crosstalk. Orthogonal promoters have been engineered utilizing an assortment of natural and synthetic transcription factors, but design of large orthogonal promoter-repressor sets is complicated, labor-intensive, and often res...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nucleic Acids Res
Egile Nagusiak: Cress, Brady F., Jones, J. Andrew, Kim, Daniel C., Leitz, Quentin D., Englaender, Jacob A., Collins, Shannon M., Linhardt, Robert J., Koffas, Mattheos A. G.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 2016
Gaiak:
Synthetic Biology and Bioengineering
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4872105/
https://ncbi.nlm.nih.gov/pubmed/27079979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw231
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