Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli

Robust gene circuit construction requires use of promoters exhibiting low crosstalk. Orthogonal promoters have been engineered utilizing an assortment of natural and synthetic transcription factors, but design of large orthogonal promoter-repressor sets is complicated, labor-intensive, and often res...

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Pubblicato in:Nucleic Acids Res
Autori principali: Cress, Brady F., Jones, J. Andrew, Kim, Daniel C., Leitz, Quentin D., Englaender, Jacob A., Collins, Shannon M., Linhardt, Robert J., Koffas, Mattheos A. G.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2016
Soggetti:
Synthetic Biology and Bioengineering
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4872105/
https://ncbi.nlm.nih.gov/pubmed/27079979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw231
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