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Orthogonal modular gene repression in E. coli using engineered CRISPR/Cas9

The progress in development of synthetic gene circuits has been hindered by the limited repertoire of available transcription factors. Recently, it has been greatly expanded using the CRISPR/Cas9 system. However, this system is limited by its imperfect DNA sequence specificity, leading to potential...

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Bibliographic Details
Published in:ACS Synth Biol
Main Authors: Didovyk, Andriy, Borek, Bartłomiej, Hasty, Jeff, Tsimring, Lev
Format: Artigo
Language:Inglês
Published: 2015
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5462464/
https://ncbi.nlm.nih.gov/pubmed/26390083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.5b00147
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