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Rapid and scalable preparation of bacterial lysates for cell-free gene expression

Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create a pr...

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Pubblicato in:ACS Synth Biol
Autori principali: Didovyk, Andriy, Tonooka, Taishi, Tsimring, Lev, Hasty, Jeff
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6038143/
https://ncbi.nlm.nih.gov/pubmed/28795570
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.7b00253
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