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Optimized Illumina PCR-free library preparation for bacterial whole genome sequencing and analysis of factors influencing de novo assembly

BACKGROUND: Next-generation sequencing (NGS) technology has paved the way for rapid and cost-efficient de novo sequencing of bacterial genomes. In particular, the introduction of PCR-free library preparation procedures (LPPs) lead to major improvements as PCR bias is largely reduced. However, in ord...

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Detalles Bibliográficos
Publicado en:BMC Res Notes
Main Authors: Huptas, Christopher, Scherer, Siegfried, Wenning, Mareike
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4864918/
https://ncbi.nlm.nih.gov/pubmed/27176120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-016-2072-9
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