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Optimizing and benchmarking de novo transcriptome sequencing: from library preparation to assembly evaluation

BACKGROUND: RNA-seq enables gene expression profiling in selected spatiotemporal windows and yields massive sequence information with relatively low cost and time investment, even for non-model species. However, there remains a large room for optimizing its workflow, in order to take full advantage...

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Bibliografiska uppgifter
I publikationen:BMC Genomics
Huvudupphovsmän: Hara, Yuichiro, Tatsumi, Kaori, Yoshida, Michio, Kajikawa, Eriko, Kiyonari, Hiroshi, Kuraku, Shigehiro
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2015
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4652379/
https://ncbi.nlm.nih.gov/pubmed/26581708
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-015-2007-1
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