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Amplicon-sequencing of raw milk microbiota: impact of DNA extraction and library-PCR
ABSTRACT: The highly complex raw milk matrix challenges the sample preparation for amplicon-sequencing due to low bacterial counts and high amounts of eukaryotic DNA originating from the cow. In this study, we optimized the extraction of bacterial DNA from raw milk for microbiome analysis and evalua...
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| Publicado en: | Appl Microbiol Biotechnol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Springer Berlin Heidelberg
2021
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8195793/ https://ncbi.nlm.nih.gov/pubmed/34059942 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-021-11353-4 |
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