Amplicon-sequencing of raw milk microbiota: impact of DNA extraction and library-PCR

ABSTRACT: The highly complex raw milk matrix challenges the sample preparation for amplicon-sequencing due to low bacterial counts and high amounts of eukaryotic DNA originating from the cow. In this study, we optimized the extraction of bacterial DNA from raw milk for microbiome analysis and evalua...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado en:Appl Microbiol Biotechnol
Autores principales: Siebert, Annemarie, Hofmann, Katharina, Staib, Lena, Doll, Etienne V., Scherer, Siegfried, Wenning, Mareike
Formato: Artigo
Lenguaje:Inglês
Publicado: Springer Berlin Heidelberg 2021
Materias:
Methods and Protocols
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8195793/
https://ncbi.nlm.nih.gov/pubmed/34059942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-021-11353-4
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares