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The impact of amplification on differential expression analyses by RNA-seq

Currently, quantitative RNA-seq methods are pushed to work with increasingly small starting amounts of RNA that require amplification. However, it is unclear how much noise or bias amplification introduces and how this affects precision and accuracy of RNA quantification. To assess the effects of am...

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Foilsithe in:Sci Rep
Main Authors: Parekh, Swati, Ziegenhain, Christoph, Vieth, Beate, Enard, Wolfgang, Hellmann, Ines
Formáid: Artigo
Teanga:Inglês
Foilsithe: Nature Publishing Group 2016
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4860583/
https://ncbi.nlm.nih.gov/pubmed/27156886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep25533
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