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The impact of amplification on differential expression analyses by RNA-seq

Currently, quantitative RNA-seq methods are pushed to work with increasingly small starting amounts of RNA that require amplification. However, it is unclear how much noise or bias amplification introduces and how this affects precision and accuracy of RNA quantification. To assess the effects of am...

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Podrobná bibliografie
Vydáno v:Sci Rep
Hlavní autoři: Parekh, Swati, Ziegenhain, Christoph, Vieth, Beate, Enard, Wolfgang, Hellmann, Ines
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4860583/
https://ncbi.nlm.nih.gov/pubmed/27156886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep25533
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