A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity

BACKGROUND: Bacterial surface display is an attractive technique for the production of cell-anchored, functional proteins and engineering of whole-cell catalysts. Although various outer membrane proteins have been used for surface display, an easy and versatile high-throughput-compatible assay for e...

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Bibliographic Details
Published in:Microb Cell Fact
Main Authors: Wendel, Sofie, Fischer, Emil C., Martínez, Virginia, Seppälä, Susanna, Nørholm, Morten H. H.
Format: Artigo
Language:Inglês
Published: BioMed Central 2016
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Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4855350/
https://ncbi.nlm.nih.gov/pubmed/27142225
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0474-y
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