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A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity
BACKGROUND: Bacterial surface display is an attractive technique for the production of cell-anchored, functional proteins and engineering of whole-cell catalysts. Although various outer membrane proteins have been used for surface display, an easy and versatile high-throughput-compatible assay for e...
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Publicado no: | Microb Cell Fact |
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Main Authors: | , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
BioMed Central
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4855350/ https://ncbi.nlm.nih.gov/pubmed/27142225 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0474-y |
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