Efficient generation of FVII gene knockout mice using CRISPR/Cas9 nuclease and truncated guided RNAs

We investigated the effects of 5′-end truncated CRISPR RNA-guided Cas9 nuclease (tru-RGN, 17/18 nucleotides) on genome editing capability in NIH/3T3 cells, and its efficiencies on generating Factor VII (FVII) gene-knockout (KO) mice. In cultured cells, RGNs on-target editing activity had been varied...

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Publicado no:Sci Rep
Main Authors: An, Liyou, Hu, Yeshu, Chang, Shiwei, Zhu, Xiumei, Ling, Pingping, Zhang, Fenli, Liu, Jiao, Liu, Yanhong, Chen, Yexiang, Yang, Lan, Presicce, Giorgio Antonio, Du, Fuliang
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4853708/
https://ncbi.nlm.nih.gov/pubmed/27139777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep25199
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