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Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Enhancers control cell identity by regulating tissue-specific gene expression in a position and orientation independent manner. These enhancers are often located distally from the regulated gene in intergenic regions or even within the body of another gene. The position independent nature of enhance...

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Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Moorthy, Sakthi D., Mitchell, Jennifer A.
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4841337/
https://ncbi.nlm.nih.gov/pubmed/27078492
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53552
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