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Quantification of transcription factor-DNA binding affinity in a living cell

The apparent dissociation constant (K(d)) for specific binding of glucocorticoid receptor (GR) and androgen receptor (AR) to DNA was determined in vivo in Xenopus oocytes. The total nuclear receptor concentration was quantified as specifically retained [(3)H]-hormone in manually isolated oocyte nucl...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Belikov, Sergey, Berg, Otto G., Wrange, Örjan
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4838337/
https://ncbi.nlm.nih.gov/pubmed/26657626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1350
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