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Quantification of transcription factor-DNA binding affinity in a living cell
The apparent dissociation constant (K(d)) for specific binding of glucocorticoid receptor (GR) and androgen receptor (AR) to DNA was determined in vivo in Xenopus oocytes. The total nuclear receptor concentration was quantified as specifically retained [(3)H]-hormone in manually isolated oocyte nucl...
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| Publicado en: | Nucleic Acids Res |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4838337/ https://ncbi.nlm.nih.gov/pubmed/26657626 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1350 |
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