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Quantification of transcription factor-DNA binding affinity in a living cell

The apparent dissociation constant (K(d)) for specific binding of glucocorticoid receptor (GR) and androgen receptor (AR) to DNA was determined in vivo in Xenopus oocytes. The total nuclear receptor concentration was quantified as specifically retained [(3)H]-hormone in manually isolated oocyte nucl...

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Bibliografski detalji
Izdano u:Nucleic Acids Res
Glavni autori: Belikov, Sergey, Berg, Otto G., Wrange, Örjan
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4838337/
https://ncbi.nlm.nih.gov/pubmed/26657626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1350
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