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Quantification of transcription factor-DNA binding affinity in a living cell
The apparent dissociation constant (K(d)) for specific binding of glucocorticoid receptor (GR) and androgen receptor (AR) to DNA was determined in vivo in Xenopus oocytes. The total nuclear receptor concentration was quantified as specifically retained [(3)H]-hormone in manually isolated oocyte nucl...
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4838337/ https://ncbi.nlm.nih.gov/pubmed/26657626 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1350 |
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