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Quantification of transcription factor-DNA binding affinity in a living cell
The apparent dissociation constant (K(d)) for specific binding of glucocorticoid receptor (GR) and androgen receptor (AR) to DNA was determined in vivo in Xenopus oocytes. The total nuclear receptor concentration was quantified as specifically retained [(3)H]-hormone in manually isolated oocyte nucl...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4838337/ https://ncbi.nlm.nih.gov/pubmed/26657626 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1350 |
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