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Mutations in human tubulin proximal to the kinesin binding site alter dynamic instability at microtubule plus- and minus-ends
The assembly of microtubule-based cellular structures depends on regulated tubulin polymerization and directional transport. Here, we purify and characterize tubulin heterodimers that have human β-tubulin isotype III (TUBB3), and heterodimers with one of two β-tubulin mutations (D417H or R262H). Bot...
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| Publicado no: | Dev Cell |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4832424/ https://ncbi.nlm.nih.gov/pubmed/27046833 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.devcel.2016.03.003 |
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