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Mismatched Primer Extension Assays

Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific mismatches at the 3′ end is a major strength of the mismatched pr...

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Bibliografski detalji
Izdano u:Bio Protoc
Glavni autori: Achuthan, Vasudevan, DeStefano, Jeffrey J.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4829118/
https://ncbi.nlm.nih.gov/pubmed/27081667
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