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A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy

PA gels have long been used as a platform to study cell traction forces due to ease of fabrication and the ability to tune their elastic properties. When the substrate is coated with an extracellular matrix protein, cells adhere to the gel and apply forces, causing the gel to deform. The deformation...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Knoll, Samantha G., Ali, M. Yakut, Saif, M. Taher A.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828080/
https://ncbi.nlm.nih.gov/pubmed/25286326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51873
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