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A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy

PA gels have long been used as a platform to study cell traction forces due to ease of fabrication and the ability to tune their elastic properties. When the substrate is coated with an extracellular matrix protein, cells adhere to the gel and apply forces, causing the gel to deform. The deformation...

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Библиографические подробности
Опубликовано в: :J Vis Exp
Главные авторы: Knoll, Samantha G., Ali, M. Yakut, Saif, M. Taher A.
Формат: Artigo
Язык:Inglês
Опубликовано: MyJove Corporation 2014
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828080/
https://ncbi.nlm.nih.gov/pubmed/25286326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51873
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