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A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy

PA gels have long been used as a platform to study cell traction forces due to ease of fabrication and the ability to tune their elastic properties. When the substrate is coated with an extracellular matrix protein, cells adhere to the gel and apply forces, causing the gel to deform. The deformation...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Knoll, Samantha G., Ali, M. Yakut, Saif, M. Taher A.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828080/
https://ncbi.nlm.nih.gov/pubmed/25286326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51873
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