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Identifying and visualizing functional PAM diversity across CRISPR-Cas systems
CRISPR-Cas adaptive immune systems in prokaryotes boast a diversity of protein families and mechanisms of action, where most systems rely on protospacer-adjacent motifs (PAMs) for DNA target recognition. Here, we developed an in vivo, positive, and tunable screen termed PAM-SCANR (PAM screen achieve...
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| Veröffentlicht in: | Mol Cell |
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| Hauptverfasser: | , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4826307/ https://ncbi.nlm.nih.gov/pubmed/27041224 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2016.02.031 |
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