Загрузка...
Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA
CRISPR-Cas9 is widely applied for genome engineering in various organisms. The assembly of single guide RNA (sgRNA) with the Cas9 protein may limit the Cas9/sgRNA effector complex function. We developed a FRET-based assay for detection of CRISPR–Cas9 complex binding to its targets and used this assa...
Сохранить в:
| Опубликовано в: : | Nucleic Acids Res |
|---|---|
| Главные авторы: | , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2016
|
| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4824121/ https://ncbi.nlm.nih.gov/pubmed/26945042 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw138 |
| Метки: |
Добавить метку
Нет меток, Требуется 1-ая метка записи!
|