A mass spectrometry-based method for direct determination of pseudouridine in RNA

Pseudouridine (5-ribosyluracil, Ψ) is the only ‘mass-silent’ nucleoside produced by post-transcriptional RNA modification. We describe here a novel mass spectrometry (MS)-based method for direct determination of Ψ in RNA. The method assigns a Ψ-containing nucleolytic RNA fragment by an accurate meas...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Yamauchi, Yoshio, Nobe, Yuko, Izumikawa, Keiichi, Higo, Daisuke, Yamagishi, Yoko, Takahashi, Nobuhiro, Nakayama, Hiroshi, Isobe, Toshiaki, Taoka, Masato
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2016
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4824092/
https://ncbi.nlm.nih.gov/pubmed/26673725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1462
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