A mass spectrometry-based method for comprehensive quantitative determination of post-transcriptional RNA modifications: the complete chemical structure of Schizosaccharomyces pombe ribosomal RNAs

We present a liquid chromatography–mass spectrometry (LC-MS)-based method for comprehensive quantitative identification of post-transcriptional modifications (PTMs) of RNA. We incorporated an in vitro-transcribed, heavy isotope-labeled reference RNA into a sample RNA solution, digested the mixture w...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Taoka, Masato, Nobe, Yuko, Hori, Masayuki, Takeuchi, Aiko, Masaki, Shunpei, Yamauchi, Yoshio, Nakayama, Hiroshi, Takahashi, Nobuhiro, Isobe, Toshiaki
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2015
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4605285/
https://ncbi.nlm.nih.gov/pubmed/26013808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv560
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