An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes

We describe here a mass spectrometry (MS)-based analytical platform of RNA, which combines direct nano-flow reversed-phase liquid chromatography (RPLC) on a spray tip column and a high-resolution LTQ-Orbitrap mass spectrometer. Operating RPLC under a very low flow rate with volatile solvents and MS...

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Bibliografske podrobnosti
Main Authors: Taoka, Masato, Yamauchi, Yoshio, Nobe, Yuko, Masaki, Shunpei, Nakayama, Hiroshi, Ishikawa, Hideaki, Takahashi, Nobuhiro, Isobe, Toshiaki
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2009
Teme:
Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2790879/
https://ncbi.nlm.nih.gov/pubmed/19740761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp732
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