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Global characterization of in vivo enzyme catalytic rates and their correspondence to in vitro k(cat) measurements

Turnover numbers, also known as k(cat) values, are fundamental properties of enzymes. However, k(cat) data are scarce and measured in vitro, thus may not faithfully represent the in vivo situation. A basic question that awaits elucidation is: how representative are k(cat) values for the maximal cata...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Davidi, Dan, Noor, Elad, Liebermeister, Wolfram, Bar-Even, Arren, Flamholz, Avi, Tummler, Katja, Barenholz, Uri, Goldenfeld, Miki, Shlomi, Tomer, Milo, Ron
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 2016
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4812741/
https://ncbi.nlm.nih.gov/pubmed/26951675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1514240113
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