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Global characterization of in vivo enzyme catalytic rates and their correspondence to in vitro k(cat) measurements

Turnover numbers, also known as k(cat) values, are fundamental properties of enzymes. However, k(cat) data are scarce and measured in vitro, thus may not faithfully represent the in vivo situation. A basic question that awaits elucidation is: how representative are k(cat) values for the maximal cata...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Davidi, Dan, Noor, Elad, Liebermeister, Wolfram, Bar-Even, Arren, Flamholz, Avi, Tummler, Katja, Barenholz, Uri, Goldenfeld, Miki, Shlomi, Tomer, Milo, Ron
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4812741/
https://ncbi.nlm.nih.gov/pubmed/26951675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1514240113
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