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Highly multiplexed targeted DNA sequencing from single nuclei

Single-cell DNA sequencing methods are challenged by poor physical coverage, high technical error rates and low throughput. To address these issues, we developed a single-cell DNA sequencing protocol that combines flow-sorting of single nuclei, time-limited multiple-displacement amplification (MDA),...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Leung, Marco L, Wang, Yong, Kim, Charissa, Gao, Ruli, Jiang, Jerry, Sei, Emi, Navin, Nicholas E
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4807405/
https://ncbi.nlm.nih.gov/pubmed/26741407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.005
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