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Application of Rigidity Theory to the Thermostabilization of Lipase A from Bacillus subtilis

Protein thermostability is a crucial factor for biotechnological enzyme applications. Protein engineering studies aimed at improving thermostability have successfully applied both directed evolution and rational design. However, for rational approaches, the major challenge remains the prediction of...

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Veröffentlicht in:PLoS Comput Biol
Hauptverfasser: Rathi, Prakash Chandra, Fulton, Alexander, Jaeger, Karl-Erich, Gohlke, Holger
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2016
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4803202/
https://ncbi.nlm.nih.gov/pubmed/27003415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1004754
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