Application of Rigidity Theory to the Thermostabilization of Lipase A from Bacillus subtilis

Protein thermostability is a crucial factor for biotechnological enzyme applications. Protein engineering studies aimed at improving thermostability have successfully applied both directed evolution and rational design. However, for rational approaches, the major challenge remains the prediction of...

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Udgivet i:PLoS Comput Biol
Main Authors: Rathi, Prakash Chandra, Fulton, Alexander, Jaeger, Karl-Erich, Gohlke, Holger
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2016
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4803202/
https://ncbi.nlm.nih.gov/pubmed/27003415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1004754
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