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Using selenomethionyl derivatives to assign sequence in low-resolution structures of the AP2 clathrin adaptor
Selenomethionine incorporation is a powerful technique for assigning sequence to regions of electron density at low resolution. Genetic introduction of methionine point mutations and the subsequent preparation and crystallization of selenomethionyl derivatives permits unambiguous sequence assignment...
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| 發表在: | Acta Crystallogr D Struct Biol |
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| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
International Union of Crystallography
2016
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4784665/ https://ncbi.nlm.nih.gov/pubmed/26960121 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2059798315021580 |
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