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Using selenomethionyl derivatives to assign sequence in low-resolution structures of the AP2 clathrin adaptor

Selenomethionine incorporation is a powerful technique for assigning sequence to regions of electron density at low resolution. Genetic introduction of methionine point mutations and the subsequent preparation and crystallization of selenomethionyl derivatives permits unambiguous sequence assignment...

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Bibliografski detalji
Izdano u:Acta Crystallogr D Struct Biol
Glavni autori: Kelly, Bernard T., Graham, Stephen C., Owen, David J.
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4784665/
https://ncbi.nlm.nih.gov/pubmed/26960121
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2059798315021580
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