A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

Currently available tools for multiplex bacterial genome engineering are optimized for a few laboratory model strains, demand extensive prior modification of the host strain, and lead to the accumulation of numerous off-target modifications. Building on prior development of multiplex automated genom...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Nyerges, Ákos, Csörgő, Bálint, Nagy, István, Bálint, Balázs, Bihari, Péter, Lázár, Viktória, Apjok, Gábor, Umenhoffer, Kinga, Bogos, Balázs, Pósfai, György, Pál, Csaba
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2016
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4780621/
https://ncbi.nlm.nih.gov/pubmed/26884157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1520040113
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