Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR)

Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5′-biotinylation of the appropriate size nucleic acids,...

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Bibliografiska uppgifter
I publikationen:J Biol Methods
Huvudupphovsmän: Kunnev, Dimiter, Freeland, Amy, Qin, Maochun, Wang, Jianmin, Pruitt, Steven C.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2015
Ämnen:
Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4778562/
https://ncbi.nlm.nih.gov/pubmed/26949711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2015.92
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