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Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR)

Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5′-biotinylation of the appropriate size nucleic acids,...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Biol Methods
Prif Awduron: Kunnev, Dimiter, Freeland, Amy, Qin, Maochun, Wang, Jianmin, Pruitt, Steven C.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2015
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4778562/
https://ncbi.nlm.nih.gov/pubmed/26949711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2015.92
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