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Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5′-biotinylation of the appropriate size nucleic acids,...
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| Udgivet i: | J Biol Methods |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4778562/ https://ncbi.nlm.nih.gov/pubmed/26949711 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2015.92 |
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