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Strand-biased cytosine deamination at the replication fork causes cytosine to thymine mutations in Escherichia coli

The rate of cytosine deamination is much higher in single-stranded DNA (ssDNA) than in double-stranded DNA, and copying the resulting uracils causes C to T mutations. To study this phenomenon, the catalytic domain of APOBEC3G (A3G-CTD), an ssDNA-specific cytosine deaminase, was expressed in an Esche...

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Bibliografische gegevens
Gepubliceerd in:Proc Natl Acad Sci U S A
Hoofdauteurs: Bhagwat, Ashok S., Hao, Weilong, Townes, Jesse P., Lee, Heewook, Tang, Haixu, Foster, Patricia L.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 2016
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4776466/
https://ncbi.nlm.nih.gov/pubmed/26839411
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1522325113
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