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High-throughput single-molecule screen for small-molecule perturbation of splicing and transcription kinetics

In eukaryotes, mRNA synthesis is catalyzed by RNA polymerase II and involves several distinct steps, including transcript initiation, elongation, cleavage, and transcript release. Splicing of RNA can occur during (co-transcriptional) or after (post-transcriptional) RNA synthesis. Thus, RNA synthesis...

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Bibliografski detalji
Izdano u:Methods
Glavni autori: Day, Christopher R., Chen, Huimin, Coulon, Antoine, Meier, Jordan L., Larson, Daniel R.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4766052/
https://ncbi.nlm.nih.gov/pubmed/26655523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2015.11.025
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