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High-throughput single-molecule screen for small-molecule perturbation of splicing and transcription kinetics

In eukaryotes, mRNA synthesis is catalyzed by RNA polymerase II and involves several distinct steps, including transcript initiation, elongation, cleavage, and transcript release. Splicing of RNA can occur during (co-transcriptional) or after (post-transcriptional) RNA synthesis. Thus, RNA synthesis...

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Publicado en:Methods
Main Authors: Day, Christopher R., Chen, Huimin, Coulon, Antoine, Meier, Jordan L., Larson, Daniel R.
Formato: Artigo
Idioma:Inglês
Publicado: 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4766052/
https://ncbi.nlm.nih.gov/pubmed/26655523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2015.11.025
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