Single amino acid substitutions uncouple the DNA binding and strand scission activities of Fok I endonuclease.
Single alanine substitution mutations at Asp-450 or Asp-467 of the type IIS restriction enzyme Fok I have no effect on the ability of the enzyme to bind strongly and selectively to its recognition site but completely eliminate its ability to cleave either strand of substrate DNA. Since wild-type Fok...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47616/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8415747/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.20.9596 |
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