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Single-molecule Force Spectroscopy Predicts a Misfolded, Domain-swapped Conformation in human γD-Crystallin Protein

Cataract is a protein misfolding disease where the size of the aggregate is directly related to the severity of the disorder. However, the molecular mechanisms that trigger the onset of aggregation remain unknown. Here we use a combination of protein engineering techniques and single-molecule force...

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Détails bibliographiques
Publié dans:J Biol Chem
Auteurs principaux: Garcia-Manyes, Sergi, Giganti, David, Badilla, Carmen L., Lezamiz, Ainhoa, Perales-Calvo, Judit, Beedle, Amy E. M., Fernández, Julio M.
Format: Artigo
Langue:Inglês
Publié: American Society for Biochemistry and Molecular Biology 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4759196/
https://ncbi.nlm.nih.gov/pubmed/26703476
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.673871
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