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Purification of Active Respiratory Supercomplex from Bovine Heart Mitochondria Enables Functional Studies

To understand the roles of mitochondrial respiratory chain supercomplexes, methods for consistently separating and preparing supercomplexes must be established. To this end, we solubilized supercomplexes from bovine heart mitochondria with digitonin and then replaced digitonin with amphipol (A8–35),...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Shinzawa-Itoh, Kyoko, Shimomura, Harunobu, Yanagisawa, Sachiko, Shimada, Satoru, Takahashi, Ryoko, Oosaki, Marika, Ogura, Takashi, Tsukihara, Tomitake
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4759192/
https://ncbi.nlm.nih.gov/pubmed/26698328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.680553
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