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Purification of Active Respiratory Supercomplex from Bovine Heart Mitochondria Enables Functional Studies
To understand the roles of mitochondrial respiratory chain supercomplexes, methods for consistently separating and preparing supercomplexes must be established. To this end, we solubilized supercomplexes from bovine heart mitochondria with digitonin and then replaced digitonin with amphipol (A8–35),...
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| Publicat a: | J Biol Chem |
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| Autors principals: | , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Biochemistry and Molecular Biology
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4759192/ https://ncbi.nlm.nih.gov/pubmed/26698328 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.680553 |
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