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A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture
Lgr5-positive stem cells can be supplemented with the essential growth factors Egf, Noggin, and R-Spondin, which allows us to culture ever-expanding primary 3D epithelial structures in vitro. Both the architecture and physiological properties of these 'mini-guts', also called organoids, cl...
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| 出版年: | J Vis Exp |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
MyJove Corporation
2014
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4758749/ https://ncbi.nlm.nih.gov/pubmed/25146755 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51765 |
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