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A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture
Lgr5-positive stem cells can be supplemented with the essential growth factors Egf, Noggin, and R-Spondin, which allows us to culture ever-expanding primary 3D epithelial structures in vitro. Both the architecture and physiological properties of these 'mini-guts', also called organoids, cl...
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| Pubblicato in: | J Vis Exp |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
MyJove Corporation
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4758749/ https://ncbi.nlm.nih.gov/pubmed/25146755 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51765 |
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