A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture

Lgr5-positive stem cells can be supplemented with the essential growth factors Egf, Noggin, and R-Spondin, which allows us to culture ever-expanding primary 3D epithelial structures in vitro. Both the architecture and physiological properties of these 'mini-guts', also called organoids, cl...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Andersson-Rolf, Amanda, Fink, Juergen, Mustata, Roxana C., Koo, Bon-Kyoung
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2014
Konular:
Genetics
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4758749/
https://ncbi.nlm.nih.gov/pubmed/25146755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51765
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