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Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates

Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in transduced target cells, from which a transduction...

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Foilsithe in:Mol Ther Methods Clin Dev
Main Authors: Murphy, Michele E, Vin, Chintan D, Slough, Megan M, Gombotz, Wayne R, Kelley-Clarke, Brenna
Formáid: Artigo
Teanga:Inglês
Foilsithe: Nature Publishing Group 2016
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4756768/
https://ncbi.nlm.nih.gov/pubmed/26942209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mtm.2016.5
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